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Bio-Techne corporation mouse il-12 p70 antibody
Mouse Il 12 P70 Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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il human il 12 miltenyi biotec  (Miltenyi Biotec)
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Il Human Il 12 Miltenyi Biotec, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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il 12  (Miltenyi Biotec)
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a , Violin plots detailing normalised XCL1 , XCL2, and CCL5 expression across annotated immune cell types in paired blood and synovial fluid (CITE-seq, n = 11). b , Frequencies (left) and geometric mean fluorescence intensities (geoMFI; right) of XCL1 + NK cells, calculated as a percentage of total NK cells (gated as live, Lin - CD3 - CD7 + events) in blood ( n = 8) and synovial fluid ( n = 7; 7 paired, 1 unpaired) following a 6-hour unstimulated culture containing brefeldin A and monensin. c , Representative flow cytometry of intracellular XCL1 in total NK cells stimulated for up to 4 hours with PMA and ionomycin, with brefeldin A and monensin. d , e , Enrichment scores for transcriptional modules associated with cytokine-stimulated NK cells ( d ) and antigen-receptor T cell activation ( e ) within NK subpopulations. f , Absolute change in XCL1 + NK cell frequency relative to unstimulated controls in paired blood and synovial fluid ( n = 8) following ex vivo stimulation with cytokines <t>(IL-12,</t> IL-15, and IL-18) or equimolar bead-conjugated activating receptor antibodies (actR; targeting CD2, CD226, CD244, NKG2D, NKp30, and NKp46). g , Quantification of XCL1 protein in paired serum and synovial fluid from patients with JIA, measured by ELISA ( n = 7). Bars indicate the mean ± s.d. Statistical significance was determined using paired two-tailed t -tests (restricted to the 7 matched pairs for b ), paired two-tailed t -tests with Benjamini-Hochberg correction ( d ), and a Wilcoxon signed-rank test following four-parameter logistic regression ( g ).
Il 12, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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il 12 23 p40 capture antibodies  (Mabtech Inc)
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a , Violin plots detailing normalised XCL1 , XCL2, and CCL5 expression across annotated immune cell types in paired blood and synovial fluid (CITE-seq, n = 11). b , Frequencies (left) and geometric mean fluorescence intensities (geoMFI; right) of XCL1 + NK cells, calculated as a percentage of total NK cells (gated as live, Lin - CD3 - CD7 + events) in blood ( n = 8) and synovial fluid ( n = 7; 7 paired, 1 unpaired) following a 6-hour unstimulated culture containing brefeldin A and monensin. c , Representative flow cytometry of intracellular XCL1 in total NK cells stimulated for up to 4 hours with PMA and ionomycin, with brefeldin A and monensin. d , e , Enrichment scores for transcriptional modules associated with cytokine-stimulated NK cells ( d ) and antigen-receptor T cell activation ( e ) within NK subpopulations. f , Absolute change in XCL1 + NK cell frequency relative to unstimulated controls in paired blood and synovial fluid ( n = 8) following ex vivo stimulation with cytokines <t>(IL-12,</t> IL-15, and IL-18) or equimolar bead-conjugated activating receptor antibodies (actR; targeting CD2, CD226, CD244, NKG2D, NKp30, and NKp46). g , Quantification of XCL1 protein in paired serum and synovial fluid from patients with JIA, measured by ELISA ( n = 7). Bars indicate the mean ± s.d. Statistical significance was determined using paired two-tailed t -tests (restricted to the 7 matched pairs for b ), paired two-tailed t -tests with Benjamini-Hochberg correction ( d ), and a Wilcoxon signed-rank test following four-parameter logistic regression ( g ).
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a , Violin plots detailing normalised XCL1 , XCL2, and CCL5 expression across annotated immune cell types in paired blood and synovial fluid (CITE-seq, n = 11). b , Frequencies (left) and geometric mean fluorescence intensities (geoMFI; right) of XCL1 + NK cells, calculated as a percentage of total NK cells (gated as live, Lin - CD3 - CD7 + events) in blood ( n = 8) and synovial fluid ( n = 7; 7 paired, 1 unpaired) following a 6-hour unstimulated culture containing brefeldin A and monensin. c , Representative flow cytometry of intracellular XCL1 in total NK cells stimulated for up to 4 hours with PMA and ionomycin, with brefeldin A and monensin. d , e , Enrichment scores for transcriptional modules associated with cytokine-stimulated NK cells ( d ) and antigen-receptor T cell activation ( e ) within NK subpopulations. f , Absolute change in XCL1 + NK cell frequency relative to unstimulated controls in paired blood and synovial fluid ( n = 8) following ex vivo stimulation with cytokines <t>(IL-12,</t> IL-15, and IL-18) or equimolar bead-conjugated activating receptor antibodies (actR; targeting CD2, CD226, CD244, NKG2D, NKp30, and NKp46). g , Quantification of XCL1 protein in paired serum and synovial fluid from patients with JIA, measured by ELISA ( n = 7). Bars indicate the mean ± s.d. Statistical significance was determined using paired two-tailed t -tests (restricted to the 7 matched pairs for b ), paired two-tailed t -tests with Benjamini-Hochberg correction ( d ), and a Wilcoxon signed-rank test following four-parameter logistic regression ( g ).
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il 12  (R&D Systems)
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a , Violin plots detailing normalised XCL1 , XCL2, and CCL5 expression across annotated immune cell types in paired blood and synovial fluid (CITE-seq, n = 11). b , Frequencies (left) and geometric mean fluorescence intensities (geoMFI; right) of XCL1 + NK cells, calculated as a percentage of total NK cells (gated as live, Lin - CD3 - CD7 + events) in blood ( n = 8) and synovial fluid ( n = 7; 7 paired, 1 unpaired) following a 6-hour unstimulated culture containing brefeldin A and monensin. c , Representative flow cytometry of intracellular XCL1 in total NK cells stimulated for up to 4 hours with PMA and ionomycin, with brefeldin A and monensin. d , e , Enrichment scores for transcriptional modules associated with cytokine-stimulated NK cells ( d ) and antigen-receptor T cell activation ( e ) within NK subpopulations. f , Absolute change in XCL1 + NK cell frequency relative to unstimulated controls in paired blood and synovial fluid ( n = 8) following ex vivo stimulation with cytokines <t>(IL-12,</t> IL-15, and IL-18) or equimolar bead-conjugated activating receptor antibodies (actR; targeting CD2, CD226, CD244, NKG2D, NKp30, and NKp46). g , Quantification of XCL1 protein in paired serum and synovial fluid from patients with JIA, measured by ELISA ( n = 7). Bars indicate the mean ± s.d. Statistical significance was determined using paired two-tailed t -tests (restricted to the 7 matched pairs for b ), paired two-tailed t -tests with Benjamini-Hochberg correction ( d ), and a Wilcoxon signed-rank test following four-parameter logistic regression ( g ).
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c r d systems cat 219 il 005 final conc 40 ng ml  (R&D Systems)
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a , Violin plots detailing normalised XCL1 , XCL2, and CCL5 expression across annotated immune cell types in paired blood and synovial fluid (CITE-seq, n = 11). b , Frequencies (left) and geometric mean fluorescence intensities (geoMFI; right) of XCL1 + NK cells, calculated as a percentage of total NK cells (gated as live, Lin - CD3 - CD7 + events) in blood ( n = 8) and synovial fluid ( n = 7; 7 paired, 1 unpaired) following a 6-hour unstimulated culture containing brefeldin A and monensin. c , Representative flow cytometry of intracellular XCL1 in total NK cells stimulated for up to 4 hours with PMA and ionomycin, with brefeldin A and monensin. d , e , Enrichment scores for transcriptional modules associated with cytokine-stimulated NK cells ( d ) and antigen-receptor T cell activation ( e ) within NK subpopulations. f , Absolute change in XCL1 + NK cell frequency relative to unstimulated controls in paired blood and synovial fluid ( n = 8) following ex vivo stimulation with cytokines <t>(IL-12,</t> IL-15, and IL-18) or equimolar bead-conjugated activating receptor antibodies (actR; targeting CD2, CD226, CD244, NKG2D, NKp30, and NKp46). g , Quantification of XCL1 protein in paired serum and synovial fluid from patients with JIA, measured by ELISA ( n = 7). Bars indicate the mean ± s.d. Statistical significance was determined using paired two-tailed t -tests (restricted to the 7 matched pairs for b ), paired two-tailed t -tests with Benjamini-Hochberg correction ( d ), and a Wilcoxon signed-rank test following four-parameter logistic regression ( g ).
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Image Search Results


a , Violin plots detailing normalised XCL1 , XCL2, and CCL5 expression across annotated immune cell types in paired blood and synovial fluid (CITE-seq, n = 11). b , Frequencies (left) and geometric mean fluorescence intensities (geoMFI; right) of XCL1 + NK cells, calculated as a percentage of total NK cells (gated as live, Lin - CD3 - CD7 + events) in blood ( n = 8) and synovial fluid ( n = 7; 7 paired, 1 unpaired) following a 6-hour unstimulated culture containing brefeldin A and monensin. c , Representative flow cytometry of intracellular XCL1 in total NK cells stimulated for up to 4 hours with PMA and ionomycin, with brefeldin A and monensin. d , e , Enrichment scores for transcriptional modules associated with cytokine-stimulated NK cells ( d ) and antigen-receptor T cell activation ( e ) within NK subpopulations. f , Absolute change in XCL1 + NK cell frequency relative to unstimulated controls in paired blood and synovial fluid ( n = 8) following ex vivo stimulation with cytokines (IL-12, IL-15, and IL-18) or equimolar bead-conjugated activating receptor antibodies (actR; targeting CD2, CD226, CD244, NKG2D, NKp30, and NKp46). g , Quantification of XCL1 protein in paired serum and synovial fluid from patients with JIA, measured by ELISA ( n = 7). Bars indicate the mean ± s.d. Statistical significance was determined using paired two-tailed t -tests (restricted to the 7 matched pairs for b ), paired two-tailed t -tests with Benjamini-Hochberg correction ( d ), and a Wilcoxon signed-rank test following four-parameter logistic regression ( g ).

Journal: bioRxiv

Article Title: Tissue-adapted NK cells shape pathogenic cDC1 niches in early arthritis

doi: 10.64898/2026.05.01.716870

Figure Lengend Snippet: a , Violin plots detailing normalised XCL1 , XCL2, and CCL5 expression across annotated immune cell types in paired blood and synovial fluid (CITE-seq, n = 11). b , Frequencies (left) and geometric mean fluorescence intensities (geoMFI; right) of XCL1 + NK cells, calculated as a percentage of total NK cells (gated as live, Lin - CD3 - CD7 + events) in blood ( n = 8) and synovial fluid ( n = 7; 7 paired, 1 unpaired) following a 6-hour unstimulated culture containing brefeldin A and monensin. c , Representative flow cytometry of intracellular XCL1 in total NK cells stimulated for up to 4 hours with PMA and ionomycin, with brefeldin A and monensin. d , e , Enrichment scores for transcriptional modules associated with cytokine-stimulated NK cells ( d ) and antigen-receptor T cell activation ( e ) within NK subpopulations. f , Absolute change in XCL1 + NK cell frequency relative to unstimulated controls in paired blood and synovial fluid ( n = 8) following ex vivo stimulation with cytokines (IL-12, IL-15, and IL-18) or equimolar bead-conjugated activating receptor antibodies (actR; targeting CD2, CD226, CD244, NKG2D, NKp30, and NKp46). g , Quantification of XCL1 protein in paired serum and synovial fluid from patients with JIA, measured by ELISA ( n = 7). Bars indicate the mean ± s.d. Statistical significance was determined using paired two-tailed t -tests (restricted to the 7 matched pairs for b ), paired two-tailed t -tests with Benjamini-Hochberg correction ( d ), and a Wilcoxon signed-rank test following four-parameter logistic regression ( g ).

Article Snippet: We performed cytokine stimulations with IL-12 (10 ng/mL, Miltenyi, 130-096-704), IL-15 (10 ng/mL, Miltenyi, 130-095-760), or IL-18 (100 ng/mL, MBL International, B001-5).

Techniques: Expressing, Fluorescence, Flow Cytometry, Activation Assay, Ex Vivo, Enzyme-linked Immunosorbent Assay, Two Tailed Test